Bisulfite Conversion

Bisulfite conversion of isolated DNA is offered using Zymo Research Corporation EZ DNA Methylation Kit or Qiagen EpiTect Bisulfite Kits. Converted DNA is suitable for PCR, endonuclease digestion, sequencing, microarrays, and Illumina Infinium and Golden Gate Methylation Assays.

Illumina Methylation Studies

Illumina recommends the following kits for bisulfite conversion. They do not recommend using any other kits.

• EZ DNA Methylation Kit for 50 DNA reactions (Zymo Research, catalog # D5001)
• EZ DNA Methylation Kit for 200 DNA reactions (Zymo Research, catalog # D5002)
• EZ-96 DNA Methylation Kit for 2x96 DNA conversion reactions (deep-well Zymo-Spin I-96 Filter Plate) (Zymo Research, catalog # D5004)

Pyromark Q24

Either type of bisulfite conversion kit may be used for the Pyromark system.

Ordering

Please contact Laura Kasch for pricing, ordering instructions and request forms.

FAF services may only be used for research purposes. Any data or samples generated by the facility may not be used for human diagnostic or therapeutic use.