Welcome
The Fragment Analysis Facility (FAF) was established in 1994 to help facilitate scientific research to the Johns Hopkins University community by providing technical assistance with genotyping and nucleic acid purification. Since then, our services have grown and are available to customers outside the JHU community.
The FAF offers genomic DNA isolation from whole blood, buffy coats, packed cells, cultured cells, blood spot cards, buccal swabs, mouthwash collection, Oragene saliva collection kits, and FFPE samples.
FAF genotyping services include SNP analysis by Taqman, fluorescent fragment analysis for determination of STR or VNTR number, analysis of insertions/deletions, and SNP discovery by sequencing.
Additional services include cell line authentication, mycoplasma testing, whole genome amplification, bisulfite conversion, DNA plating, DNA storage, and PCR support for Pyrosequencing offered through the GRCF DNA Analysis Facility.
The FAF is located off-campus approximately 1.5 miles from the Johns Hopkins Medical campus and maintains a drop off site at the hospital.
News
Cell Line Authentication
- New reporting format for cell line authentication. Receive an individual signed report for each cell line submitted that contains a STR profile table and a graphic profile (electropherogram) for your submitted line.
- FAF now offers a new level of STR profiling: comparison of your line's profile to the ATCC STR Profiling database, DSMZ database and the JCRB database.
- The PowerPlex 1.2 System kit is no longer offered due to discontinuation by the manufacturer. Either of the two other kits we use to generate an STR profile gives the same information, plus additional information, and is suitable for database searches.
- Coming soon: New web based ordering system.
Mycoplasma Testing
- New lower mycoplasma testing prices.
- New reporting format for mycoplasma testing. You will receive an individual report for each cell line submitted.
- Coming soon: New web based ordering system.
FAF services may only be used for research purposes. Any data or samples generated by the facility may not be used for human diagnostic or therapeutic use.