Nucleic Acid Purification
The FAF offers genomic DNA purification from whole blood, buffy coat, packed cells, cultured cells, blood spot cards, FFPE samples, and buccal swabs/brushes, mouthwash, and Oragene saliva collection kits. Depending on sample type, purification is carried out with either Puregene kit reagents (Qiagen) on an Autopure automated workstation or Qiagen column based kits on a QIAcube automated workstation. A260 and A280 measurements are determined with a Nanodrop spectrophotometer.
DNA is suitable for archiving, PCR, Southern blotting, library construction and other applications requiring high molecular weight DNA.
Prices range from $25 to $45 per sample. Volume pricing is available for large study projects or batched samples.
Ordering
Please place your order through our new secure online ordering system. Click on "Create New Account" to create a login.
For assistance filling out the online request please call 443-287-7948 or view our DNA Isolation Ordering Instructions.
Please contact Laura Kasch at 410-614-3830 or lkasch@jhmi.edu for pricing
Delivery of samples
JH Customers: Drop samples off in Blalock 1004 with the GeneSifter order number, Monday - Friday from 9 AM - 4:00 PM.
External Customers: Send the samples with the GeneSifter order number to:
Laura Kasch
Fragment Analysis Facility
Johns Hopkins University
2760 Lighthouse Point East, Suite 201
Baltimore, MD 21224
Phone (443) 287-7948
Samples should be sent for delivery Monday through Friday only.