Nucleic Acid Purification
The FAF offers genomic DNA purification from whole blood, buffy coats, cultured cells, blood clots, packed cells, FFPE samples, and buccal, mouthwash and saliva samples. Purification is carried out using Puregene kit reagents (Gentra Systems) and a Autopure automated workstation. Samples sent for processing are logged into a database, checking samples against a request form provided by the client, isolated using a non-organic method, and have A260 and A280 readings determined using a Beckman DU530 spectrophotometer. Samples are returned to the client or may be stored at the FAF.Average DNA yield from one ml blood is 33µg. Average DNA yield from one buccal swab is 1-2µg. DNA is suitable for PCR, Southern blotting, library construction and other applications requiring high molecular weight DNA.The FAF has over 15 years experience using Puregene chemistry for DNA purifiacation.
Protocol
Blood should be collected in EDTA (lavender top) vacutainers. Up to 10ml of blood can be extracted as one isolation sample. A FAF request form must be submitted with samples. The FAF will not accept samples with subject name identifiers. It is the responsibility of the investigator to supply a unique identifier.
Request Form
Genomic DNA Isolation Form (.pdf)