Whole Genome Amplification

Whole genome amplification of extracted DNA is carried out using a REPLI-g kit (Qiagen). The kit uses Multiple Displacement Amplification technology to produce highly uniform amplification across the genome. The genome is isothermally amplified utilizing a DNA polymerase with 3' - 5' exonuclease proofreading activity capable of replicating up to 100 kb without disassociating from the DNA template. Typical yields from the REPLI-g kit are 40ug DNA with a product range of 2 to 100 kb and an average of greater than10 kb. Poor quality genomic dna may result in synthesis failure due to an inhibitor in sample, reduced call rates, or loss of loci or alleles in the amplification product.

Ordering

Please contact Laura Kasch for pricing, ordering instructions and request forms.

FAF services may only be used for research purposes. Any data or samples generated by the facility may not be used for human diagnostic or therapeutic use.